Changes in contrast sensitivity after surgery for intermittent exotropia

To determine whether intermittent exotropia (IXT) surgery affects contrast sensitivity (CS), this retrospective study evaluated the changes in monocular and binocular CS and the binocular summation ratio (BSR) quantified as the ratio between the values of the binocular and the better monocular CS score (BSR = binocular CS score/better monocular CS score) after surgery for IXT. The subjects were patients who had undergone IXT-correcting surgery with a postoperative follow-up of > 3 months and had pre- and postoperative records of Mars CS test scores. In total, 64 patients (128 eyes) were evaluated. Both the binocular and monocular CS scores of the operated eyes were significantly worse on postoperative 1 day than the preoperative scores, but they were recovered after 1 week. The monocular CS scores of the operated eyes were significantly worse than those of the non-operated eyes until 1 week. There was no significant difference in monocular CS scores between the one-muscle and two-muscle surgeries and in binocular CS scores between the successful alignment and overcorrection groups even on the first day after surgery. The mean BSR was significantly decreased until postoperative month 1, however, recovered to preoperative levels after month 3. In conclusion, IXT-correcting surgery may temporarily worsen the CS, but it is recovered to preoperative levels. Thus, changes in CS in the immediate postoperative period after strabismus surgery should not be of concern.


Changes in contrast sensitivity after surgery for intermittent exotropia Young Hwan Bae & Dong Gyu Choi *
To determine whether intermittent exotropia (IXT) surgery affects contrast sensitivity (CS), this retrospective study evaluated the changes in monocular and binocular CS and the binocular summation ratio (BSR) quantified as the ratio between the values of the binocular and the better monocular CS score (BSR = binocular CS score/better monocular CS score) after surgery for IXT. The subjects were patients who had undergone IXT-correcting surgery with a postoperative follow-up of > 3 months and had pre-and postoperative records of Mars CS test scores. In total, 64 patients (128 eyes) were evaluated. Both the binocular and monocular CS scores of the operated eyes were significantly worse on postoperative 1 day than the preoperative scores, but they were recovered after 1 week. The monocular CS scores of the operated eyes were significantly worse than those of the non-operated eyes until 1 week. There was no significant difference in monocular CS scores between the one-muscle and two-muscle surgeries and in binocular CS scores between the successful alignment and overcorrection groups even on the first day after surgery. The mean BSR was significantly decreased until postoperative month 1, however, recovered to preoperative levels after month 3. In conclusion, IXT-correcting surgery may temporarily worsen the CS, but it is recovered to preoperative levels. Thus, changes in CS in the immediate postoperative period after strabismus surgery should not be of concern.
Visual performance is mainly measured by letter charts, such as the Snellen chart that uses high-contrast black letters on a white background. However, some individuals with good visual acuity (VA) in the Snellen chart test at a very high contrast may be visually handicapped in real-life situations. Therefore, measuring other factors, including contrast sensitivity (CS), color vision, or glare, may be helpful for a more accurate assessment of the quality of vision 1-3 . The CS test represents the spatial resolution of the visual system, which can be abnormal in many diseases including amblyopia, optic neuritis, cataract, glaucoma, strabismus, and brain lesion 4,5 . Several studies have investigated CS in exotropia patients under different conditions, such as the presence or absence of glare and degree of illumination [6][7][8][9] . However, to date, only a limited number of studies have evaluated the changes in monocular and binocular CS according to surgical outcomes after strabismus surgery.
Therefore, this study aimed to determine whether intermittent exotropia (IXT) surgery affects CS scores. We investigated the changes in the monocular and binocular CS, and the binocular summation, which was defined as the superiority of the binocular over monocular performance after surgery for IXT 10 . In addition, the CS scores were analyzed according to the surgical outcomes (successful alignment versus overcorrection) and surgical methods for correcting IXT.

Measurement of CS scores and quantification of the binocular summation ratio (BSR). The CS
was assessed using the Mars contrast sensitivity test (The Mars Perceptrix Corporation, Chappaqua, NY, USA). Briefly, the Mars test is used to measure spatial CS at peak sensitivity. It is a handheld chart with a sequence of 48 randomly ordered 10 different letters. Measurements to the right eye, left eye, and both eyes are performed individually with three separate charts with the best optical corrections. Each chart has eight lines with six letters per line with a letter size of 6/145 Snellen, which is approximately 0.8 cycles per degree. In this study, the Mars test was performed by one of three trained technicians with a luminance of approximately 90 cd/m 2 and 50 cm from the patient. The logarithmic value was used for the analysis of CS, which was defined as "CS scores". Patients were encouraged to get the correct answer with each correct letter representing an accumulation of 0.04 log units. The test ended when two consecutive letter-reading errors were made, and the final score was the log CS of the last successful letter, with a decrement of 0.04, for any wrong answers that precede the two continuous errors 11,12 . Using CS scores, the BSR was quantified as the ratio between the values of the binocular and the better monocular CS score. (BSR = binocular CS score/better monocular CS score).

Strabismus surgery and postoperative management. Bilateral lateral rectus muscle recession (BLR)
or unilateral lateral rectus recession-medial rectus resection (R&R) was performed under general anesthesia by a single surgeon (DGC) who had no preference for either procedure. Unilateral lateral rectus recession (ULR) was commonly performed for exotropia of ≤ 25 PD at distance and near. The surgical dosage was based on the distant angle of exodeviation, as shown in Table 1. The surgical methods were decided by the surgeon; however, surgeries were performed after discussion with the patients or their parents about one-eye or two-eye surgery and one-muscle or two-muscle surgery in 20-25 PD exotropia.
Eye alignment at distance and near fixation in the primary position was measured at 1 day, 1 week, 1 month, and 3 months postoperatively. Patients who had esodeviation or diplopia postoperatively were prescribed alternate full-time patching until the disappearance of diplopia or esodeviation. If esotropia or diplopia persisted by 2 months, cycloplegic refraction was rechecked, the residual or recurrent hyperopia was corrected, and base-out Fresnel press-on prisms (3M Press-On OpticsTM; 3M Health Care, St Paul, MN, USA) were prescribed until the esotropia disappeared.
Outcome measures. The main outcome measures were (1) the postoperative changes in BSR, monocular and binocular CS scores in the overall population, (2) the difference in the monocular CS scores between the operated and non-operated eyes, (3) the difference in the monocular CS scores of operated eyes between the eyes with one-muscle surgery (unilateral rectus recession) and two-muscle surgery (R&R), (4) the difference in binocular CS scores between the successful alignment group (defined as an alignment between 10 PD exodeviation and 5 PD esodeviation at distance and near) and overcorrection group (defined as > 5 PD esodeviation at  Statistical analysis. The Mann-Whitney U test, Spearman correlation test, and Kruskal-Wallis test were used to analyze the association between preoperative demographic data and CS scores. A paired t test was used to compare between the preoperative and postoperative CS scores and BSR. The Mann-Whitney U test and Spearman correlation test were used to analyze the correlation of the postoperative binocular CS score and BSR with the postoperative sensory status and exodeviation. An independent samples t test was used to analyze monocular CS scores according to surgical methods and binocular CS scores according to the postoperative deviation. All statistical analyses were performed using SPSS software, version 25 (SPSS Inc., Chicago, IL, USA). A P value of < 0.05 was considered statistically significant.

Results
In total, 64 patients (128 eyes) were included in the study. The clinicodemographic patient characteristics and their statistical correlations with the preoperative CS scores are presented in Table 2. There were 35 men and 29 women, and the mean age at surgery was 12.18 ± 9.73 years (range, 2.33-64.42). The mean preoperative VA in logMAR was 0.04 ± 0.07 (range, 0-0.40), and the mean spherical equivalent was − 1.69 ± 2.22D (range, − 11.0 to + 1.88D). The mean preoperative angle of deviation was 24.53 ± 7.61 PD (range, 15-50 PD) at distance and 28.26 ± 9.43 PD (range, 6-55 PD) at near. Good stereopsis (≤ 100 s of arc) in Titmus stereotest was achieved in 71.9%, and fusion in the Worth-4-dot test was achieved in 37.5%. Of the 64 patients, R&R was performed in 47 patients, ULR in 14 patients, and BLR in 3 patients. Among the preoperative characteristics, stereopsis measured by Titmus stereotest was the only factor significantly associated with the CS score (P = 0.032, Mann-Whitney U test). Patients with good stereopsis (defined as 100 arcsec or better in Titmus test) had significantly better binocular CS scores. The mean preoperative BSR was 1.015 ± 0.030, which significantly decreased at postoperative day 1 (1.006 ± 0.028) and month 1 (1.006 ± 0.025) (P < 0.05); however, it recovered to preoperative levels after month 3 (1.016 ± 0.022). The mean binocular CS score (logarithmic scales) was significantly lower at postoperative day 1 than at the preoperative period (1.72 ± 0.05 vs 1.75 ± 0.04, P < 0.001). However, it was recovered to preoperative levels after 1 week (P > 0.05). Similarly, the monocular CS score of the operated eyes was also significantly lower at postoperative day 1 than at the preoperative period (1.65 ± 0.81 vs 1.71 ± 0.06, P < 0.001) and was recovered to preoperative levels after 1 week (P > 0.05). Meanwhile, there was no significant change in the mean monocular CS score of the non-operated eyes (P > 0.05) ( Table 3).
The mean monocular CS score of the operated eyes was significantly worse than that of non-operated eyes at postoperative day 1 and week 1; however, the difference was no longer significant starting from 1 month postoperatively (Fig. 1). There was no significant difference in binocular CS scores between the successful alignment group and overcorrection group on every visit (Fig. 2, P > 0.05). Further, there was also no significant difference www.nature.com/scientificreports/ in monocular CS scores from the first day after surgery to the last visit between the eyes with one-muscle surgery (unilateral rectus recession) (n = 14 eyes) and two-muscle surgery (R&R) (n = 47 eyes) (Fig. 3, P > 0.05).
In the correlation analysis of the binocular CS score with the clinical characteristics at postoperative month 3, the mean binocular CS score was significantly higher in patients with good stereopsis (100 arcsec of better) (P < 0.05); however, it did not correlate with the exodeviation angles (P > 0.05). In the correlation analysis of the BSR with the deviation angles and sensory status, no correlation was observed (Table 4). Meanwhile, preoperatively, the proportions "good stereopsis" on the Titmus test and "Fusion" on Worth-4-dot test were 89.06% and 37.50%, respectively, which temporarily decreased at postoperative day 1 to 59.38% and 17.19%, respectively, and gradually recovered to the preoperative level until 3 months (90.64% and 37.50%).

Discussion
CS refers to the visual ability to detect differences in luminance between an object and its background 13,14 . Several instruments, including the Contrast Glare tester 2000 (CGT-2000; Takagi Seiko, Nagano, Japan), Optec 6500 vision testing system (Stereo Optical Co., Inc., Chicago, IL, USA), and CSV 1000 (Vector Vision, Dayton OH), have been recently used to measure CS under different spatial frequencies, luminance, and presence or absence of glare [6][7][8][9]15,16 . However, their clinical use is restricted because of their limited availability and the time-consuming measurements. In this study, we used the widely used Mars contrast sensitivity test, which has good validity and reasonable agreement with the Pelli-Robson chart 11,17,18 . The Mars CS test can be used relatively easily and quickly in clinical practice, and its clinical results have been published in several studies 15,19,20 . Current guidelines in Table 3. Comparison between pre-and postoperative mean binocular and monocular CS scores. *P < 0.05, significant changes compared to preoperative values (paired t test). Significant values are in bold.  Figure 1. Comparison of the monocular CS scores between operated eyes (n = 71) and non-operated eyes (n = 65). *Statistically significant difference between the 2 groups using the independent samples t test (P < 0.05).  17,18 . In this study, the mean preoperative binocular CS score  www.nature.com/scientificreports/ was 1.75 ± 0.04, and the mean monocular CS score of the operated eyes and non-operated eyes was 1.71 ± 0.06. These results are similar to those of previous reports 17,18 , although all subjects had intermittent exotropia. Kim et al. reported an improvement in binocular CS scores under photopic conditions at almost all visual angles after surgery for IXT 7 . However, in our study, the binocular CS score decreased at postoperative day 1 but then recovered to the preoperative level after 1 week.

Monocular CS scores Operated eyes (n = 71) Non-operated eyes (n = 65)
Kwon et al. reported that a poorer degree of stereopsis was associated with lower CS, and better fusional ability was associated with higher CS under mesopic conditions 8 . Therefore, the CS test is helpful for evaluating sensory function in IXT. Our study showed similar results; patients with good stereopsis (defined as 100 arcsec or better on the Titmus test) had significantly better binocular CS scores. At the postoperative month 3, the binocular CS scores and proportion of patients with 'good stereopsis' and fusion on Worth-4-dot recovered to the preoperative levels and patients with good stereopsis obtained statistically higher binocular CS scores, which suggest that the change in the sensory status, especially stereopsis, could have an effect on the changes in the postoperative CS scores.
In the correlation analysis for preoperative monocular CS scores of 128 eyes, distance VA was not statistically correlated with the CS scores. Considering the test distance of Mars contrast sensitivity test (50 cm), the analysis should be performed with the near VA. However, we routinely checked the Snellen VA at 5 m at every visit, and the correlation of the CS scores was analyzed with distant VA because of the retrospective nature of this study. Several studies have reported that CS worsened after ocular surgeries, such as laser in situ keratomileusis and macula-on rhegmatogenous retinal detachment, which might be due to decreased central VA after surgery 16,21,22 . However, even patients with good postoperative VA have decreased CS, and this could possibly be due to factors other than VA such as surgical stress and visual discomfort 23 . CS is temporarily decreased after refractive surgeries but is recovered after 3 months 16 .
The corneal stromal remodeling and wound healing process after refractive surgeries would contribute to diffusing light rays passing through the cornea, resulting in decreased subjective measures of CS 16 . In our study, the mean binocular CS score and the monocular CS score of the operated eyes were significantly decreased at postoperative day 1. However, there was no significant difference between the pre-and post-operative values of CS in non-operated eyes. The monocular CS score of the operated eyes was significantly worse than that of the non-operated eyes until 1 week, but it returned to the baseline level after 1 month. CS score recovery to preoperative levels was faster in IXT surgery than in refractive surgery. Moreover, there was no significant difference in monocular CS scores between the eyes with one-muscle surgery and those with two-muscle surgery, even on the first day after surgery. Therefore, the worsening of CS scores during the short-term postoperative period may have been caused by other factors instead of the corneal structural change in cases of refractive surgeries. These factors include surgical stress or difficulty in opening the eyes due to foreign body sensation or epiphora caused by sutured knots, discomfort in conjunctival incision sites, and chemosis, which would have occurred temporarily after surgery although the test was performed after irrigation if there was some discharge interrupting the measurement 24,25 . This study had some limitations. First, as it was a retrospective study, and the reproducibility and repeatability of the CS scores were not assessed. Second, our study included both children and older adult subjects, leading to some bias in the interpretation of the results of the CS scores. However, the statistical analysis using the Spearman correlation test showed that the preoperative CS scores were not affected by age at surgery (P = 0.389). Finally, a comparison with normal controls could not be performed. Further prospective clinical studies are needed to evaluate the reproducibility and repeatability of the test with a larger number of subjects. However, this study is meaningful in that it measured and analyzed monocular and binocular CS four times after IXT surgery using Mars test which is repeatable, is low cost and time efficient, and a standardized scoring method for assessing CS.
In conclusion, surgery for intermittent exotropia does not affect CS measurement in the long-term. Thus, changes in CS in the immediate postoperative period after strabismus surgery should not be of concern.

Data availability
The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.  Table 4. Correlation binocular contrast sensitivity (CS) score and binocular summation ratio with sensory status and deviation angles at postoperative month 3. PD prism diopters. *Mann-Whitney U test, † Spearman correlation test. Significant values are in bold.

Sensory status
Deviation angles (PD)